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OCR: VIRAL SUPERNATANT Viral titer (on 3T3 cells) Sterilir Mvcoplasma Injection into mice Viral testing MAP test 3T3 amplification +L assay (ecotropic xenotropic and ampho- tropic Electron microscopy TRANSDUCED TUMOR-INFII TRATING LYMPHOCYTES 3T3 amplification assay Reverse transcriptase assay Endotoxin assay IL-2 withdrawal G418 selection Southern blot or PCR to detect inserted gene NPT assay Cytotoxic profile Phenotypic profile PATIENT Test cells from patient for virus (3T3 amplification plus . minus assay Serum test for antibody tc heiper virus antigen 4070A (Western blo() VIRAI Sterility Mycoplasma INFILTRATING withdrawa amplifcarion mins