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PSION CD 2
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1068
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TheRealBuffer.sis
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EPOC Installation Package
|
2001-09-29
|
8KB
|
249 lines
C:\System\Apps\therealbuffer\TheRealBufferc:\system\apps\TheRealBuffer\TheRealBufferC:\System\Apps\therealbuffer\TheRealBuffer.aifc:\system\apps\TheRealBuffer\TheRealBuffer.aifC:\System\Apps\therealbuffer\TheRealBuffer.appc:\system\apps\TheRealBuffer\TheRealBuffer.appTheRealBufferP
"DATA.APP
Table1
ColA1
ColB1
ColA2
ColB2
ColA3
ColB3
ColA4
ColB4
ColA5
ColB5
ColA6
ColB6
ColA7
ColB7
H^jJTVbPXPXRZRPTU
Maleate
Maleic acid
116.1U Phosphate
0.0044
Phosphoric acid
98.0U
Chloroacetate
0.0023
Chloroacetic acid
94.5U
Formate
Formic acid
46.03U
Benzoate
0.018
Benzoic acid
122.1U
Acetate
-0.0002
Acetic acid
60.05U
Propionate
-0.0002
Propionic acid
74.05U
Pyridine
-0.014
Pyridine
79.10U
Piperazine
-0.015
Piperazine
86.14U
-0.011
MES free acid
195.2U
Bis-tris
-0.02
Bis-tris base
209.2U
-0.011
ADA free acid
190.2U
PIPES
-0.0085
PIPES free acid
302.4U
-0.02
ACES free acid
182.2U
-0.016
BES free acid
213.3U
-0.011
MOPS free acid
209.3
Table1
Name:
"dpKa/dt:
Ions:
*Substance:
Note:
\PXt`\R\TV\ZjU Phosphate
-0.0028
e.g. NaH2PO4
142.0U
-0.02
TES free acid
229.25U
HEPES
-0.014
HEPES free acid
238.3U
-0.028
Tris base
121.4
binds to metall ionsU
Tricine
-0.021
Tricine free acid
179.2U
Bicine
-0.018
Bicine free acid
163.2U
-0.02
TAPS free acid
243.3U
Ethanolamine
-0.029
Ethanolamine
61.1U
-0.018
CHES free acid
207.3U
10.51
-0.018
CAPS free acid
221.3U
Methylamine
10.62
-0.031
Methylamine
31.1U
Piperidine
11.12
-0.031
Piperidine
85.15U Phosphate
12.33
-0.026
e.g. Na2HPO4-7H2O
268.07;@O
Buffer
Puffer
*c:\Programme\The Real Buffer\TheRealBuffer.
+*c:\system\apps\TheRealBuffer\TheRealBuffer
BUFFER$
SUBST$
*W [H
BUFFER$#
Set...O
Buffer,pKa
Ok O
Choose buffer parametersO
Buffer:
Concentration [mM] O
pH-Value: O
Ok O
Back O
Choose Buffer parametersO
Buffers pKa:
Concentration [mM] O
pH-Value: O
Ok O
Back O
More parametersO
Volume [ml]: O
Use at [
C]: O
Make at [
C]: O
Ok O
Back O
BUFFER$#
SUBST$#
+.The pH is not suitable for the choosen buffer!+*Please select a new buffer (or pka) or pH.+
Ok W8
+$Do you like to set a ionic strength?O
Enter: +
No,Yes
Ionic strength [mM]: O
Choose Salt: +
NaCl,KCl,Na2SO4,other
Ok O
*fffffFM@R
g NaClK
g KClK
g Na2SO4K
salt to
MolarK
+AIonic strength due to buffer species exeeds needed ionic stength!+
Please enter new values...+
Ok W8
9@NRJ
no salt
*ffffff
You prepare
ml of
buffer of pH
in the lab
C and use it at
C. The thermodynamic pKa is
and the
apparent pKa' will be
|RWB}*
+ . The temperature coefficient is
Dissolve
'|RWB}*
mole acid and
'|RWB}*
mole basic buffer component.
. Make up to
ml with destilled water.
Alternatively, dissolve
|RWB}*
approx.
ml destilled water. Add
titrate to pH
Od|RWB}*
C with HCl or NaOH. Then make up
ml with distilled water.
press any key to continue...
You prepare
ml of
buffer of pH
in the lab
C and use it at
C. The thermodynamic pKa is
and the
apparent pKa' will be
|RWB}*
+ . The temperature coefficient is
Dissolve
'|RWB}*
mole acid and
'|RWB}*
mole basic buffer component.
. Make up to
ml with destilled water.
Alternatively, dissolve
|RWB}*
approx.
ml destilled water. Add
titrate to pH
Od|RWB}*
C with HCl or NaOH. Then make up
ml with distilled water.
press any key to continue...
Start again?
Yes O
No O
TheRealBuffer
Thank you for using the programO
Dr. R
bbe W
nschiersO
rw@biowasserstoff.deO
release 2.1O
BUFFER?