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The Datafile PD-CD 5
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DATAFILE_PDCD5.iso
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rasmol
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antibody
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!Antibody
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IGG-FAB2_S
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1996-03-03
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2KB
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80 lines
#PUT UP A SOLID GREEN IGG1
zap
script igg1-a.scr
color green
spacefill
script clrleg.scr
echo Figure 3-7 (p. 46, Abbas, 2nd ed) shows whole IgG molecules.
echo Here is an Fab dimer, an antibody missing its Fc arm.
echo An antigen sees a solid surface like this, but also
echo sees opportunities for hydrogen bonding, charges,
echo and hydrophobic patches.
pause
#ZOOM DOWN TO ALLOW FASTER ROTATION
zoom 80
echo
echo This is a good size for faster rotation.
script wave.scr
pause
#COLOR THE CHAINS NICELY
color chain
select :l
color cyan
select :h
color orange
select all
script clrleg.scr
echo Light chains are light colors, heavy chains, dark.
echo (Rotate at this size.)
script wave.scr
pause
#ZOOM UP
zoom 185
script clrleg.scr
echo Here are the chains separately colored, full-screen size again.
pause
#STICKS
spacefill off
backbone off
wireframe 0.15
script clrleg.scr
echo The STICK or WIREFRAME display has too much detail.
echo The S-S bonds are buried in the molecule.
pause
#RIBBONS
wireframe off
ribbons on
script clrleg.scr
echo This CARTOON or RIBBON display emphasizes the immuno-
echo globluin (Ig) domains, 4 constant and 4 variable.
pause
#SHOW THE BACKBONE AND DISULFIDES
ribbons off
backbone 0.3
script clrleg.scr
echo The BACKBONE representation also shows the structure
echo clearly, and allows much faster rotation.
script wave.scr
pause
#ZOOM IN ON THE HINGE REGION
script igg1pose.scr
translate y -50
zoom 500
script clrleg.scr
echo Two S-S bonds link the heavy chains at the hinge.
echo Each light chain is linked to its heavy chain with
echo . an S-S bond.
script wave1.scr
pause
script igg1pose.scr
zoom 100
pause