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1996-01-30
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Document 0166
DOCN M9610166
TI [Bacteriological diagnosis of tuberculosis: current hieratic
classification of methods]
DT 9601
AU Carbonnelle B; Carpentier E; Laboratoire de bacteriologie-virologie, CHU
d'Angers, France.
SO Rev Med Interne. 1995;16(7):518-23. Unique Identifier : AIDSLINE
MED/96008889
AB To assure the diagnosis of tuberculosis, one needs the observation, the
isolation and the identification of the causative agent Mycobacterium
tuberculosis. In this approach, the microscopic exam occurs as a fast
but neither sensitive or specific test. The isolation on solid media is
slow and needs more than three weeks before becoming positive.
Nevertheless, it is a sensitive and specific one. The identification of
the isolated strain and the study of sensitivity to antibiotic agents
require an equal delay. Then, 2 months are necessary to achieve the
analysis. The AIDS epidemic with the increase of opportunistic
mycobacterial diseases, and the unexpected arrival of resistant
Mycobacteria is creating as a difficult therapeutic problem. The
cultivation in liquid media with the radiometric method (Bactec)
shortens the time of culture by half. The genomic amplification assay
has been hopeful because it allowed results in 2 days. However, some
technical difficulties happen when the test is conducted and it is less
sensitive than the isolation process. The hierarchical classification of
the laboratory useful process to establish the diagnosis of tuberculosis
disease remains the microscopic observation of the bacilli and their
isolation. Today, the use of PCR alone does not assure the diagnosis of
tuberculosis, however it may be used as a additional diagnostic test.
DE AIDS-Related Opportunistic Infections/*DIAGNOSIS Bacteriological
Techniques/*CLASSIFICATION English Abstract Female Human Male
Mycobacterium tuberculosis/ISOLATION & PURIF Polymerase Chain Reaction
Tuberculosis/*DIAGNOSIS/ETIOLOGY/MICROBIOLOGY JOURNAL ARTICLE REVIEW
REVIEW, TUTORIAL
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).