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M9610384.TXT
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1996-01-30
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Document 0384
DOCN M9610384
TI Characterization of Streptococcus pneumoniae from human immunodeficiency
virus--seropositive patients with acute and recurrent pneumonia.
DT 9601
AU Jordens JZ; Paul J; Bates J; Beaumont C; Kimari J; Gilks C; Public
Health Laboratory, John Radcliffe Hospital, Headington,; Oxford, United
Kingdom.
SO J Infect Dis. 1995 Oct;172(4):983-7. Unique Identifier : AIDSLINE
MED/96029364
AB Thirty-two isolates of clinically significant Streptococcus pneumoniae
from 11 human immunodeficiency virus (HIV)-seropositive patients with
single or multiple episodes of pneumonia were characterized by
antibiotic susceptibility testing, serotyping, ribotyping, and
repetitive extragenic palindromic polymerase chain reaction (REP-PCR).
The isolates comprised 10 serotypes, 12 ribotyping patterns, and 12
REP-PCR patterns. There was close but not absolute correlation between
techniques. By combining these characterization methods, 14 strains were
identified. Five strains were found in > 1 patient, suggesting their
frequent occurrence in this population. Two isolates of different
serotype from 1 patient were highly related by ribotyping and REP-PCR,
suggesting possible in vivo serotype change. Acute infection was
associated with single strains or coinfection by distinct strains.
Recurrent pneumonia was identified as relapse with the same strain or
reinfection with new strains. The molecular characterization of
pneumonococci from HIV-seropositive persons refines our understanding of
pneumonococcal infection in these patients.
DE Acute Disease Adult Bacterial Typing Techniques DNA,
Ribosomal/GENETICS Human HIV Seropositivity/*COMPLICATIONS
Kenya/EPIDEMIOLOGY Microbial Sensitivity Tests Pneumonia,
Lobar/*COMPLICATIONS/*EPIDEMIOLOGY Polymerase Chain Reaction
Recurrence Serotyping Streptococcus
pneumoniae/*CLASSIFICATION/GENETICS Support, Non-U.S. Gov't JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).