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M9610507.TXT
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1996-01-30
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Document 0507
DOCN M9610507
TI Diagnosis of Toxoplasma gondii infection in AIDS patients by a
tissue-culture technique.
DT 9601
AU Contini C; Romani R; Magno S; Delia S; Institute of Infectious and
Respiratory Diseases, University of; Ferrara, Italy.
SO Eur J Clin Microbiol Infect Dis. 1995 May;14(5):434-40. Unique
Identifier : AIDSLINE MED/96039821
AB Toxoplasma gondii infection was investigated in 14 AIDS patients with
neurological involvement who showed clinical and computer tomographic
scan signs suggestive of toxoplasmic encephalitis or extraneuronal
localization suggestive of toxoplasmic infection. Blood, lung secretion
(bronchoalveolar lavage and/or induced sputum sample) and cerebral
spinal fluid (CSF) samples were cultured, and the results compared with
the results of direct examination by Giemsa and by immunofluorescence of
lung secretions and CSF. Toxoplasma gondii were observed directly in
only four patients by immunofluorescence in bronchoalveolar lavage,
induced sputum and CSF samples, and in three of these patients by Giemsa
staining of bronchoalveolar lavage and CSF smears. In contrast,
parasites were detected after 48 h in blood cultures from 11 of the 14
AIDS patients, in CSF cultures from eight of them and also in cultures
of bronchoalveolar lavage and induced sputum from the six patients with
respiratory and radiological features indicative of lung tissue damage.
The findings indicate the value of tissue culture for diagnosis of
toxoplasmosis as well as for monitoring the effects of treatment and
also indicate that culture of induced sputum could be a powerful tool in
establishing the incidence of pulmonary toxoplasmosis in AIDS patients.
DE Acquired Immunodeficiency Syndrome/COMPLICATIONS Adult Animal
AIDS-Related Opportunistic Infections/BLOOD/CEREBROSPINAL FLUID/
*DIAGNOSIS/PARASITOLOGY Bronchoalveolar Lavage Fluid/PARASITOLOGY
Female Fluorescent Antibody Technique Human Male Sputum/PARASITOLOGY
Support, Non-U.S. Gov't Tissue Culture Toxoplasma/*ISOLATION & PURIF
Toxoplasmosis/BLOOD/CEREBROSPINAL FLUID/COMPLICATIONS/*DIAGNOSIS
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).