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M9610554.TXT
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1996-01-30
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Document 0554
DOCN M9610554
TI Examination of variables affecting syncytium formation by, and serum
neutralization of, feline immunodeficiency virus on CrFK cells.
DT 9601
AU Bandecchi P; Pistello M; Matteucci D; Lombardi S; Bendinelli M; Tozzini
F; Department of Animal Pathology, University of Pisa, Italy.
SO New Microbiol. 1995 Jul;18(3):241-52. Unique Identifier : AIDSLINE
MED/96028320
AB The feline immunodeficiency virus (FIV) induces syncytia in Crandell
feline kidney (CrFK) cells grown in low fetal bovine serum-containing
medium. This finding has allowed the development of sensitive FIV
titration and neutralization assays using syncytium formation as an
indicator of infection. In this report we examine several variables that
can influence number and size of syncytia. In addition, by performing
assays under rigidly controlled culture conditions, we confirm that
serum neutralization assays based on FIV-induced syncytium formation in
CrFK cells detect broadly reactive neutralizing antibodies.
DE Animal Antibodies, Viral/*IMMUNOLOGY Carbon Dioxide/PHARMACOLOGY Cats
Cell Line Culture Media Giant Cells/*METABOLISM/PATHOLOGY/VIROLOGY
Hydrogen-Ion Concentration Immune Sera/IMMUNOLOGY Immunodeficiency
Virus, Feline/*GROWTH & DEVELOPMENT/GENETICS/ IMMUNOLOGY
Neutralization Tests/*METHODS Polymerase Chain Reaction Sodium
Bicarbonate/PHARMACOLOGY Support, Non-U.S. Gov't Time Factors JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).