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M9620243.TXT
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1996-02-26
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Document 0243
DOCN M9620243
TI Eimeria acervulina infection: evidence for the involvement of CD8+ T
lymphocytes in sporozoite transport and host protection.
DT 9602
AU Trout JM; Lillehoj HS; USDA, Agricultural Research Service, Beltsville,
Maryland 20705,; USA.
SO Poult Sci. 1995 Jul;74(7):1117-25. Unique Identifier : AIDSLINE
MED/96075754
AB Host-parasite interactions during coccidial infection are complex, and
host responses leading to parasite elimination are not well understood.
Interactions between host cells and parasites were investigated using
two-color immunofluorescent staining of duodenal tissue infected with
Eimeria acervulina. Tissues were removed from TK strain chickens 24, 48,
and 72 h after primary infection with 2.5 x 10(7) normal or irradiated
oocysts and also following secondary infection with 2.5 x 10(7) normal
oocysts. The tissues were embedded in freezing compound, frozen,
sectioned, and fixed in cold acetone. Sections were stained with
monoclonal antibodies to identify CD4+, CD8+, Ig+, and Bu1a+
lymphocytes, as well as macrophages and parasites. At 24 h after all
infections, sporozoites were seen primarily in CD8+ lymphocytes and
macrophages. Many CD8+ lymphocytes were seen at all times in infected
tissues and frequently appeared to be in contact with infected
epithelial cells. Sporozoites were occasionally seen in CD4+ lymphocytes
but none were noted in Ig+ or Bu1a+ lymphocytes. Meront development was
extensive after primary infection with normal oocysts, sparse after
primary infection with irradiated oocysts, and moderate after secondary
infection.
DE Animal Antibodies, Monoclonal *Chickens
Coccidiosis/IMMUNOLOGY/PHYSIOPATHOLOGY/*VETERINARY CD8-Positive
T-Lymphocytes/*IMMUNOLOGY Eimeria/*IMMUNOLOGY Host-Parasite Relations
Kinetics Photomicrography Poultry Diseases/IMMUNOLOGY/*PARASITOLOGY
Support, U.S. Gov't, Non-P.H.S. Time Factors JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).