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- Document 0400
- DOCN M9620400
- TI Polypyrimidine tract-binding protein and heterogeneous nuclear
- ribonucleoprotein A1 bind to human T-cell leukemia virus type 2 RNA
- regulatory elements.
- DT 9602
- AU Black AC; Luo J; Watanabe C; Chun S; Bakker A; Fraser JK; Morgan JP;
- Rosenblatt JD; Division of Hematology/Oncology, University of
- California, Los; Angeles 90095-1678, USA.
- SO J Virol. 1995 Nov;69(11):6852-8. Unique Identifier : AIDSLINE
- MED/96013782
- AB Efficient expression of human T-cell leukemia virus (HTLV) and human
- immunodeficiency virus structural proteins requires Rx and Rev proteins,
- respectively. Decreased expression of Gag and Env appears to be due, in
- part, to intragenic RNA sequences, termed cis-acting repressive
- sequences (CRS), and may be mediated by binding of specific cellular
- factors. We demonstrated previously that two cellular proteins, p60CRS
- and p40CRS, interact with HTLV type 2.5' long terminal repeat CRS RNA
- and that the interaction of both proteins with CRS RNA correlates with
- function (A. C. Black, C. T. Ruland, J. Luo, A. Bakker, J. K. Fraser,
- and J. D. Rosenblatt, Virology 200:29-41, 1994). By
- radioimmunoprecipitation of HeLa nuclear proteins UV cross-linked to CRS
- RNAs with murine monoclonal antibodies, we now show that p40CRS is
- heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and p60CRS is
- polypyrimidine tract-binding protein or hnRNP I. These
- immunoprecipitation results were confirmed by an immunobinding assay
- with hnRNP I and hnRNP AI antibodies and by cross-competition
- electrophoretic mobility shift experiments. In addition, we mapped a
- putative hnRNP A1 binding site in U5 RNA and demonstrated that p40CRS
- (hnRNP A1) binding to that site correlates with CRS function. Since both
- hnRNP I and hnRNP A1 have been shown to influence splicing and
- potentially other steps in RNA processing, the binding of both hnRNP I
- and hnRNP A1 to HTLV RNA regulatory elements may alter retrovirus RNA
- processing and may be involved in regulation by Rex.
- DE Base Sequence Binding Sites Chloramphenicol
- Acetyltransferase/BIOSYNTHESIS Gene Expression Gene Expression
- Regulation, Viral Gene Products, env/BIOSYNTHESIS Gene Products,
- gag/BIOSYNTHESIS Human HTLV-II/GENETICS/*PHYSIOLOGY Molecular
- Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides
- Oligonucleotides, Antisense *Regulatory Sequences, Nucleic Acid
- Ribonucleoproteins/*METABOLISM RNA-Binding Proteins/*METABOLISM
- Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transfection
- JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
-
-