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1996-02-26
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Document 0427
DOCN M9620427
TI Total number of immunocompetent cells in the normal rat epididymis and
after vasectomy.
DT 9602
AU Hooper P; Smythe E; Richards RC; Howard CV; Lynch RV; Lewis-Jones DI;
Department of Human Anatomy and Cell Biology, University of; Liverpool,
UK.
SO J Reprod Fertil. 1995 Jul;104(2):193-8. Unique Identifier : AIDSLINE
MED/96046541
AB The aim of this study was to make unbiased quantitative estimates of the
number of T8 cytotoxic cells and T4 helper cells and macrophages in the
head and tail of rat epididymides, using newly developed design-based
stereological methods in fertile control animals, and to compare these
with results from vasectomized animals. The location of these cells
within the intra- and interepithelial compartments of the epididymis was
also studied. In control animals, both the T4 helper cells and
macrophages and T8 cytotoxic cells were found in significantly higher
numbers (P < 0.01) in the head interepithelial location than in the
intraepithelial site. In addition, there were significantly more (P <
0.01) T8 cytotoxic cells in the tail interepithelial region. Within the
head region of vasectomized rats, there was a significant increase in T8
cytotoxic cells in the intraepithelial location (P < 0.01), and a
decrease in T4 helper cells and macrophages in the interepithelial
region (P < 0.05). Serum samples from vasectomized animals, when
compared with those from control animals, showed a significant increase
in cytotoxic antisperm antibody. This suggests that, in response to the
increased spermatozoal antigenic absorption that takes place in the
epididymis after vasectomy, the immune system redistributes its T cell
subsets to modify the immune tolerance that may occur.
DE Animal CD4 Lymphocyte Count CD4-Positive T-Lymphocytes/CYTOLOGY
CD8-Positive T-Lymphocytes/CYTOLOGY Epididymis/CYTOLOGY/*IMMUNOLOGY
Immunocompetence Immunohistochemistry Lymphocyte Count
Macrophages/*CYTOLOGY Male Rats Rats, Wistar Spermatozoa/CYTOLOGY
Support, Non-U.S. Gov't T-Lymphocytes/*CYTOLOGY *Vasectomy JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).