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M9620536.TXT
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1996-02-26
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Document 0536
DOCN M9620536
TI Processing of exogenous heat-aggregated (denatured) and particulate
(native) hepatitis B surface antigen for class I-restricted epitope
presentation.
DT 9602
AU Schirmbeck R; Bohm W; Melber K; Reimann J; Institute for Medical
Microbiology, University of Ulm, Germany.
SO J Immunol. 1995 Nov 15;155(10):4676-84. Unique Identifier : AIDSLINE
MED/96062283
AB Many cell types efficiently present an epitope of the hepatitis B
surface Ag (HBsAg) to murine class I-restricted CTL following an in
vitro pulse with native 22-nm HBsAg particles. Processing of exogenous
HBsAg particles required its cytochalasin B-insensitive uptake and acid
proteolysis in an endocytic compartment, was insensitive to brefeldin A
and cycloheximide, and did not involve regurgitation of antigenic
peptides. In contrast, after an in vitro pulse of cells with exogenous,
heat-denatured 1-micron HBsAg aggregates, only macrophages (but not
other cell types tested) presented the Ld-restricted HBsAg epitope
efficiently to CTL. Processing of exogenous HBsAg aggregates required
its cytochalasin B-sensitive uptake, was insensitive to brefeldin A, and
involved regurgitation of antigenic peptides. Processing of the two
different, exogenous HBsAg preparations for class I-restricted epitope
presentation thus involved alternative pathways: an endocytic pathway
for native 22-nm particles, and a phagocytic pathway for denatured
1-microns aggregates. Both HBsAg preparations displayed different
immunogenicity for class I-restricted CTL in vivo when delivered without
adjuvants: native HBsAg particles were of high immunogenicity, and
denatured HBsAg aggregates were of low immunogenicity. Class
I-restricted CTL are thus primed in vivo after endocytic processing of
native HBsAg particles as well as phagocytic processing of denatured
HBsAg aggregates.
DE Animal *Antigen Presentation Cells, Cultured CD8-Positive
T-Lymphocytes/*IMMUNOLOGY Endocytosis/IMMUNOLOGY Heat Hepatitis B
Antigens/CHEMISTRY/*IMMUNOLOGY Histocompatibility Antigens Class
I/*IMMUNOLOGY Mice Mice, Inbred BALB C Phagocytosis/IMMUNOLOGY
Recombinant Proteins/CHEMISTRY/IMMUNOLOGY Support, Non-U.S. Gov't
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).