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M9620679.TXT
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1996-02-26
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Document 0679
DOCN M9620679
TI Assessment of aerosol containment on the ELITE flow cytometer.
DT 9602
AU Ferbas J; Chadwick KR; Logar A; Patterson AE; Gilpin RW; Margolick JB;
Department of Infectious Diseases and Microbiology, Graduate; School of
Public Health, University of Pittsburgh, Pennsylvania,; USA.
SO Cytometry. 1995 Mar 15;22(1):45-7. Unique Identifier : AIDSLINE
MED/96090282
AB Biohazardous aerosols generated during cell sorting have been of
increased concern recently because of interest in sorting specimens
containing human immunodeficiency virus type 1 (HIV-1). Current flow
cytometers have features designed to contain such aerosols within the
sorting chamber, but the efficacy of these features has not been
established. Therefore, we tested aerosol containment by two ELITE flow
cytometers (Coulter Cytometry, Inc., Hialeah, FL) during sorting of
specimens containing high titers of bacteriophage. Agar plates confluent
with susceptible Escherichia coli were used to detect infectious units
released from the sorting chamber. Under recommended operating
conditions very few infectious units were released from the sorting
chambers. Release increased when the center stream was not optimally
collected in a vacuum-exhausted tube or the chamber door was not
completely closed. Failure of the negative pressure and high efficiency
particle air (HEPA) filtration features had less of an effect. The data
indicate that these standard safety features provide a rational
expectation of safety for the flow cytometry operator.
DE Aerosols Cell Separation/INSTRUMENTATION *Containment of Biohazards
Evaluation Studies Flow Cytometry/*INSTRUMENTATION Human
HIV-1/ISOLATION & PURIF Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).