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- Document 0874
- DOCN M9620874
- TI Ribosomal internal transcribed spacer sequences are identical among
- Mycobacterium avium-intracellulare complex isolates from AIDS patients,
- but vary among isolates from elderly pulmonary disease patients.
- DT 9602
- AU De Smet KA; Brown IN; Yates M; Ivanyi J; MRC Tuberculosis and Related
- Infections Unit, Hammersmith; Hospital, London, UK.
- SO Microbiology. 1995 Oct;141 ( Pt 10):2739-47. Unique Identifier :
- AIDSLINE GENBANK/X74056
- AB Sequencing 280 bp of the internal transcribed spacer (ITS) between the
- 16S and 23S rRNA genes in a collection of 46 clinical isolates of the
- Mycobacterium avium-intracellulare complex (MAI complex) identified nine
- different sequences, grouping these isolates in nine 'ITS sequevars'.
- This analysis extends the subdivision within the MAI complex to 18 ITS
- sequevars and also improves discrimination from other mycobacterial
- species. Evaluation of the sequevar grouping among different clinical
- sources revealed strong association of the M. avium sequevar Mav-B with
- AIDS and with lymphadenitis in children (18 out of 20 and 3 out of 3
- respectively). Isolates from elderly patients with pulmonary disease and
- not suspected of being HIV infected belonged predominantly to M.
- intracellulare ITS sequevars and sequevars not assigned to either M.
- avium or M. intracellulare. On the other hand, animal isolates were of
- both the Mav-A and Mav-B sequevars. We conclude that ITS sequevar typing
- is an accurate way of identifying distinct MAI complex strains. The
- observed differences between clinical sources suggest that ITS sequevars
- reflect possibly important, biologically and clinically relevant
- polymorphisms between MAI complex organisms.
- DE Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY Aged
- Animal Base Sequence Child Comparative Study DNA, Bacterial/GENETICS
- DNA, Ribosomal/*GENETICS Genes, Bacterial Human Lung
- Diseases/COMPLICATIONS/*MICROBIOLOGY Molecular Sequence Data
- Mycobacterium avium/GENETICS Mycobacterium avium
- Complex/CLASSIFICATION/*GENETICS/IMMUNOLOGY Mycobacterium
- avium-intracellulare Infection/COMPLICATIONS/ *MICROBIOLOGY Phylogeny
- RNA, Ribosomal, 16S/GENETICS Sequence Analysis, DNA Sequence Homology,
- Nucleic Acid Serotyping Tuberculosis/VETERINARY JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
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