home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Collection of Education
/
collectionofeducationcarat1997.iso
/
HEALTH
/
MED9602.ZIP
/
M9620874.TXT
< prev
next >
Wrap
Text File
|
1996-02-26
|
3KB
|
42 lines
Document 0874
DOCN M9620874
TI Ribosomal internal transcribed spacer sequences are identical among
Mycobacterium avium-intracellulare complex isolates from AIDS patients,
but vary among isolates from elderly pulmonary disease patients.
DT 9602
AU De Smet KA; Brown IN; Yates M; Ivanyi J; MRC Tuberculosis and Related
Infections Unit, Hammersmith; Hospital, London, UK.
SO Microbiology. 1995 Oct;141 ( Pt 10):2739-47. Unique Identifier :
AIDSLINE GENBANK/X74056
AB Sequencing 280 bp of the internal transcribed spacer (ITS) between the
16S and 23S rRNA genes in a collection of 46 clinical isolates of the
Mycobacterium avium-intracellulare complex (MAI complex) identified nine
different sequences, grouping these isolates in nine 'ITS sequevars'.
This analysis extends the subdivision within the MAI complex to 18 ITS
sequevars and also improves discrimination from other mycobacterial
species. Evaluation of the sequevar grouping among different clinical
sources revealed strong association of the M. avium sequevar Mav-B with
AIDS and with lymphadenitis in children (18 out of 20 and 3 out of 3
respectively). Isolates from elderly patients with pulmonary disease and
not suspected of being HIV infected belonged predominantly to M.
intracellulare ITS sequevars and sequevars not assigned to either M.
avium or M. intracellulare. On the other hand, animal isolates were of
both the Mav-A and Mav-B sequevars. We conclude that ITS sequevar typing
is an accurate way of identifying distinct MAI complex strains. The
observed differences between clinical sources suggest that ITS sequevars
reflect possibly important, biologically and clinically relevant
polymorphisms between MAI complex organisms.
DE Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY Aged
Animal Base Sequence Child Comparative Study DNA, Bacterial/GENETICS
DNA, Ribosomal/*GENETICS Genes, Bacterial Human Lung
Diseases/COMPLICATIONS/*MICROBIOLOGY Molecular Sequence Data
Mycobacterium avium/GENETICS Mycobacterium avium
Complex/CLASSIFICATION/*GENETICS/IMMUNOLOGY Mycobacterium
avium-intracellulare Infection/COMPLICATIONS/ *MICROBIOLOGY Phylogeny
RNA, Ribosomal, 16S/GENETICS Sequence Analysis, DNA Sequence Homology,
Nucleic Acid Serotyping Tuberculosis/VETERINARY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).