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1996-02-26
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Document 0886
DOCN M9620886
TI Short-term in vitro culture and molecular analysis of the
microsporidian, Enterocytozoon bieneusi.
DT 9602
AU Visvesvara G; Leitch GJ; Pieniazek NJ; Da Silva AJ; Wallace S; Slemenda
SB; Weber R; Schwartz DA; Gorelkin L; Wilcox CM; et al; Division of
Parasitic Diseases, National Center for Infectious; Diseases, Centers
for Disease Control and Prevention, Public; Health Service, U.S.
Department of Health and Human Services,; Atlanta, Georgia 30341-3724,
USA.
SO J Eukaryot Microbiol. 1995 Sep-Oct;42(5):506-10. Unique Identifier :
AIDSLINE MED/96010583
AB The microsporidium, Enterocytozoon bieneusi, causes a severe,
debilitating, chronic diarrhea in patients with the acquired
immunodeficiency syndrome. Specific diagnosis of intestinal
microsporidiosis, especially due to Enterocytozoon, is difficult and
there is no known therapy that can completely eradicate this parasite.
Preliminary studies indicate that a short term (about 6 months) in vitro
culture of this parasite yielding low numbers of spores, may be
established by inoculating human lung fibroblasts and/or monkey kidney
cell cultures with duodenal aspirates and or biopsy from infected
patients. The cultures may subsequently be used for the isolation and
molecular analysis of parasite DNA.
DE Adult Animal AIDS-Related Opportunistic
Infections/DIAGNOSIS/PARASITOLOGY Base Sequence Cells, Cultured
Cercopithecus aethiops DNA, Protozoan/GENETICS DNA, Ribosomal/GENETICS
Fibroblasts Human Lung Male Microspora
Infections/DIAGNOSIS/*PARASITOLOGY Microsporida/*GROWTH &
DEVELOPMENT/*GENETICS/ISOLATION & PURIF/ ULTRASTRUCTURE Middle Age
Molecular Sequence Data Parasitology/METHODS RNA, Ribosomal/*GENETICS
Sequence Analysis, DNA Support, U.S. Gov't, P.H.S. Vero Cells JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).