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- Document 1076
- DOCN M9621076
- TI Characteristics of perforin expressing lymphocytes within the first
- trimester decidua of human pregnancy.
- DT 9602
- AU Rukavina D; Rubesa G; Gudelj L; Haller H; Podack ER; Department of
- Physiology and Immunology, Medical Faculty,; University of Rijeka,
- Croatia.
- SO Am J Reprod Immunol. 1995 May;33(5):394-404. Unique Identifier :
- AIDSLINE MED/96070201
- AB PROBLEM: The number of perforin (P)-positive cells in decidua of
- pregnancy is larger than that observed in any other pathological
- condition. The aim was to investigate the distribution and the phenotype
- of P+ cells. METHOD: Decidual tissue was obtained from the first
- trimester vaginal termination of pregnancy. Tissue distribution of P+
- cells was analyzed by immunohistochemistry. The method for simultaneous
- measurement of P and cell surface is presented. RESULTS: There is no
- difference in number and distribution of P+ cells between decidua
- basalis (DB) and decidua parietalis (DP). The percentage of P+ decidual
- lymphocytes (DL) is two times higher than in peripheral blood
- lymphocytes (PBL) (55% vs. 27%), and the prevalent phenotype is CD3-
- CD4- CD8- CD2+ (95%) CD11c+ (68%) and CD56+ (82%). CD56bright+ DL are
- also Pbright+ and this is the largest DL subpopulation (42.4% DL). Two
- different subpopulations of CD8+ DL exist: 1) CD8bright+, which are CD3+
- CD56- P- and 2) CD8dim+, which are CD3- CD56+ P+. CONCLUSION: P
- expressing DL are prevalently nonclassical NK cells (CD16-) with low
- cytolytic activity but fully equipped with potent cytolytic machinery
- (Pbright+). There are no classical cytotoxic lymphocytes (CTL) (CD3+
- CD8+ P+) in the decidua, and all CD8+ P+ cells are CD3- CD56+. The
- number of P+ cells is even higher in DP in the vicinity of noninvasive
- trophoblast, than in DB.
- DE Antigens, CD56/ANALYSIS Antigens, CD8/ANALYSIS Biological
- Markers/ANALYSIS CD8-Positive T-Lymphocytes/CHEMISTRY
- Decidua/*IMMUNOLOGY Female Human Immunophenotyping Killer Cells,
- Natural/CHEMISTRY Membrane Glycoproteins/*ANALYSIS Pregnancy
- Pregnancy Trimester, First/*IMMUNOLOGY Staining Support, Non-U.S.
- Gov't T-Lymphocytes/*CHEMISTRY JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
-
-