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Document 0149
DOCN M9610149
TI Dysregulation of T helper cell cytokines in autoimmune prone NZB x NZW
F1 mice.
DT 9601
AU Lin LC; Chen YC; Chou CC; Hsieh KH; Chiang BL; Department of Pediatrics,
College of Medicine, National Taiwan; University, China.
SO Scand J Immunol. 1995 Oct;42(4):466-72. Unique Identifier : AIDSLINE
MED/96017848
AB Multifactorial involvement in the pathogenesis of autoimmune NZB/W F1
mice has been well documented. To further elucidate the role of
cytokines in the disease development of murine lupus, single spleen
cells isolated from NZB/W F1 and non-autoimmune C57BL/6 mice were
stimulated with T cell mitogens or anti-CD3 antibody at pre-determined
optimal concentration. Supernatants were collected and assayed for
production of cytokines including IL-2, gamma-IFN, IL-3, IL-4, IL-5 and
IL-10. In both young and old mice, cytokine profiles by
mitogen-stimulated T cells showed higher TH2 (type 2 T helper)
cell-related cytokine production in NZB/W F1 mice compared to those in
non-autoimmune C57BL/6 mice. In contrast, cytokines produced by TH1
(type 1 T helper) cells, such as gamma-IFN and IL-2, were lower in NZB/W
F1 mice by stimulation with either mitogen or anti-CD3 antibody. In
addition, cytokine production at different time points also demonstrated
decreased gamma-IFN and increased IL-4 levels by anti-CD3 stimulated
splenic cells in autoimmune NZB/W F1 mice. Furthermore, the IL-10 levels
produced by lipopolysaccharide (LPS)-stimulated splenic and peritoneal
exudate cells were higher in young NZB/W F1 mice compared to those in
C57BL/6 mice. Our data suggest that dysregulation between TH1 and TH2
cells may play an important role in the pathogenesis of autoimmunity in
NZB/W F1 mice.
DE Age Factors Animal Autoimmunity/*IMMUNOLOGY Cells, Cultured
Cytokines/*BIOSYNTHESIS/IMMUNOLOGY Female Immunophenotyping Mice
Mice, Inbred C57BL Mice, Inbred NZB Spleen/IMMUNOLOGY Support,
Non-U.S. Gov't Th1 Cells/*IMMUNOLOGY/METABOLISM Th2
Cells/*IMMUNOLOGY/METABOLISM JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).