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- Document 0179
- DOCN M9620179
- TI Both immunization with protein and recombinant vaccinia virus can
- stimulate CTL specific for the E7 protein of human papilloma virus 16 in
- H-2d mice.
- DT 9602
- AU Zhu X; Tommasino M; Vousden K; Sadovnikava E; Rappuoli R; Crawford L;
- Kast M; Melief CJ; Beverley PC; Stauss HJ; MCR Tuberculosis and Related
- Infections Unit, Royal Postgraduate; Medical School, Hammersmith
- Hospital, London, UK.
- SO Scand J Immunol. 1995 Nov;42(5):557-63. Unique Identifier : AIDSLINE
- MED/96062122
- AB The transforming protein E7 of human papilloma virus type 16 can
- stimulate cytotoxic T lymphocytes (CTL) which can protect experimental
- animals against growth of E7 expressing tumour cells. In this study we
- compared CTL responses in mice immunized with either E7 protein in MF59
- adjuvant or with recombinant vaccinia virus expressing E7 (Vac-E7). We
- have chosen H-2d mice because no E7-specific CTL responses have been
- described in this MHC haplotype. Immunization of these mice with Vac-E7
- generated CTL which lysed target cells infected with Vac-E7 or
- transfected with the E7 gene. CTL from mice immunized with E7 protein in
- MF59 adjuvant showed specificity for the same target cells. Antibody
- blocking experiments revealed that both immunization with Vac-E7 and E7
- protein stimulated CD8+ effector CTL. The find specificity of CTL
- induced by the two immunization protocols was similar. A major CTL
- epitope was mapped to the carboxyl terminal amino acids 48-98 of the E7
- protein. Peptide isolation from E7 expressing cells followed by HPLC
- separation indicated that CTL induced by immunization with protein and
- Vac-E7 recognized the same HPLC purified peptide fractions. Together,
- the study suggests that vaccines based on protein can activate CTL with
- similar fine specificity to CTL induced by vaccines based on recombinant
- vaccinia virus.
- DE Adjuvants, Immunologic Amino Acid Sequence Animal Cytotoxicity,
- Immunologic CD8-Positive T-Lymphocytes/*IMMUNOLOGY Epitopes,
- T-Lymphocyte/IMMUNOLOGY Mice Mice, Inbred BALB C Mice, Inbred DBA
- Molecular Sequence Data Oncogene Proteins, Viral/*IMMUNOLOGY
- Peptides/CHEMISTRY/IMMUNOLOGY T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
- Vaccines, Synthetic/*IMMUNOLOGY Vaccinia Virus Viral
- Vaccines/*IMMUNOLOGY JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
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-