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Document 0373
DOCN M9620373
TI [ELISA for diagnosis of infections by viruses]
DT 9602
AU Yoshihara N; AIDS Research Center, National Institute of Health.
SO Nippon Rinsho. 1995 Sep;53(9):2277-82. Unique Identifier : AIDSLINE
MED/96008330
AB The assay used most widely to detect or diagnose virus infection,
especially infection of blood borne viruses e.g. HBV, HCV, HIV and HTLV,
is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and
practicability have rendered it the most common primary screening assay.
ELISA can be mass screening used automatic or semiautomatic machines.
ELISAs can be indirect assay, competition assays or sandwich assays. In
indirect and sandwich assays, the development of color indicates the
presence of antigen or antibody, whereas in competition assays the
absence of color development signifies a positive reaction. Alkaline
phosphatase and horseradish peroxidase the most commonly used enzymes,
are associated with their respective substrates, usually p-nitro-phenyl
phosphate and hydrogen peroxide. The ELISA for antigen detection is used
polyclonal antibody or monoclonal antibodies. The other hand, the ELISA
for antibody screening is used whole virus, synthetic peptides or
recombinant antigens.
DE Acquired Immunodeficiency Syndrome/DIAGNOSIS English Abstract
*Enzyme-Linked Immunosorbent Assay/METHODS Hepatitis B/DIAGNOSIS
Hepatitis C/DIAGNOSIS Human HTLV-BLV Infections/DIAGNOSIS Virus
Diseases/*DIAGNOSIS JOURNAL ARTICLE REVIEW REVIEW, TUTORIAL
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).