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1996-02-26
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Document 0504
DOCN M9620504
TI Identification of virulence-associated antigens and plasmids in
Rhodococcus equi from patients with AIDS.
DT 9602
AU Takai S; Imai Y; Fukunaga N; Uchida Y; Kamisawa K; Sasaki Y; Tsubaki S;
Sekizaki T; Department of Animal Hygiene, School of Veterinary Medicine
and; Animal Sciences, Kitasato University, Towada, Japan.
SO J Infect Dis. 1995 Nov;172(5):1306-11. Unique Identifier : AIDSLINE
MED/96036388
AB Rhodococcus equi is an emerging opportunistic pathogen of human
immunodeficiency virus-infected patients. However, little is known about
the characteristics of R. equi isolates from humans. This study
characterized the plasmid content, expression of a virulence-associated
antigen, and mouse virulence of 19 R. equi isolates from patients with
and without AIDS. EcoRI digestion patterns and Southern, Western, and
virulence analyses of these isolates with cryptic plasmids allowed
definition of a new category of R. equi. Isolates from patients with
AIDS tended either to be virulent and have 15- to 17-kDa antigens and an
85-kb plasmid (10(6) bacteria needed for lethality) or have intermediate
virulence (10(7) bacteria needed for lethality) and one of four distinct
large plasmids that share DNA homology and express a 20-kDa antigen.
Most of the non-AIDS isolates were avirulent (> 10(8) bacteria needed
for lethality) and did not express any of these antigens.
DE Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Actinomycetales
Infections/COMPLICATIONS/*MICROBIOLOGY/VETERINARY Animal Antibodies,
Monoclonal AIDS-Related Opportunistic Infections/*MICROBIOLOGY
Blotting, Southern Blotting, Western DNA Probes Electrophoresis,
Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Female Horse
Diseases Horses Human Mice Mice, Inbred BALB C/IMMUNOLOGY *Plasmids
Restriction Mapping Rhodococcus equi/*GENETICS/ISOLATION &
PURIF/*PATHOGENICITY Support, Non-U.S. Gov't Virulence JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).