home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Collection of Education
/
collectionofeducationcarat1997.iso
/
HEALTH
/
MED9605D.ZIP
/
M9650978.TXT
< prev
next >
Wrap
Text File
|
1996-03-30
|
3KB
|
50 lines
Document 0978
DOCN M9650978
TI Kinetics of ex-vivo cytokine production by splenocytes during murine
acquired immunodeficiency syndrome (MAIDS).
DT 9505
AU Akarid K; Pocidalo MA; Desforges B; Sinet M; INSERM U 13, Hopital
Bichat-Claude Bernard, Paris, France.
SO Eur Cytokine Netw. 1995 May-Jun;6(3):181-5. Unique Identifier : AIDSLINE
MED/96159499
AB The role of T helper 1 (Th1) and T helper 2 (Th2) responses in the
murine acquired immunodeficiency syndrome (MAIDS) is unclear. It has
been suggested that differential activation of T cell subsets,
particularly a shift to Th2 cytokine production, may be associated with
disease progression. To clarify the regulation of the cytokine network
in the course of MAIDS, we examined the kinetics of cytokine production
by isolated splenocytes. C57/BL6 mice were infected with the LP-BM5
mixture. The spleen cell proliferative response, together with IL-2,
IFN-gamma, IL-10 and IL-4 production by unstimulated and ConA or
anti-CD3 MoAb-stimulated spleen cells, were determined at various times
after inoculation (weeks 1, 3, 6 and 9). Spleen cells isolated from
murine leukemia virus complex (LP-BM5) infected mice spontaneously
produced significant amounts of IL-2 and IFN-gamma one and three weeks
post-infection, compared to uninfected controls. The capacity of
isolated T cells to produce the Th1 cytokines IL-2 and IFN-gamma in
response to stimulation with ConA and anti-CD3 MoAb decreased after 3
weeks of infection. The fall in IL-2 production ran parallel to the fall
in the T cell proliferative response to ConA. IL-10 production in
response to ConA and anti-CD3 MoAb increased after three weeks
post-inoculation, and followed the reverse kinetic pattern to IFN-gamma
and IL-2. In contrast, no significant spontaneous IL-4 production and no
increase in IL-4 production in response to ConA or anti-CD3 MoAb
occurred during the course of MAIDS, relative to uninfected controls.
These results suggest that LP-BM5 infection leads to a fall in Th1
cytokine production rather than a clear switch to Th2 cytokine
production.
DE Animal Cells, Cultured Concanavalin A/PHARMACOLOGY
Cytokines/*BIOSYNTHESIS/GENETICS Female Gene Expression
Regulation/DRUG EFFECTS Interferon Type II/BIOSYNTHESIS/GENETICS
Interleukin-10/BIOSYNTHESIS/GENETICS
Interleukin-2/BIOSYNTHESIS/GENETICS Interleukin-4/BIOSYNTHESIS/GENETICS
Kinetics Lymphocyte Transformation Mice Mice, Inbred C57BL
Mitogens/PHARMACOLOGY Murine Acquired Immunodeficiency
Syndrome/*PATHOLOGY Muromonab-CD3/PHARMACOLOGY
Spleen/*METABOLISM/PATHOLOGY Th1 Cells/*METABOLISM Th2
Cells/*METABOLISM JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).