home *** CD-ROM | disk | FTP | other *** search
- Document 0520
- DOCN M9610520
- TI Novel glycolipids of Mycobacterium avium and related M. paratuberculosis
- strains of relevance to AIDS and Crohn's disease.
- DT 9601
- AU Ohene-Gyan KA; Haagsma J; Davies MJ; Hounsell EF; Department of
- Bacteriology, Central Veterinary Institute,; Lelystad, The Netherlands.
- SO Comp Immunol Microbiol Infect Dis. 1995 Jun;18(3):161-70. Unique
- Identifier : AIDSLINE MED/96002248
- AB The polar glycolipid fractions of several mycobacterial strains of the
- closely related species M. avium and M. paratuberculosis have been
- analysed by thin layer chromatography (TLC), high pH anion exchange
- chromatography (HPAEC), gas-liquid chromatography (GC) and nuclear
- magnetic resonance (NMR) spectroscopy. The upper phase of a Folch
- partitioning (rather than the lower phase analysed by others) was
- subjected to TLC in solvent system chloroform-methanol-water 50:40:10
- v/v/v. A major band was purified from each mycobacterial strain.
- Monosaccharide analysis of that from M. avium A14 (from an AIDS patient)
- contained Glc, Ara, Man, Gal in ratios 7:4:3:2. whereas one strain of M.
- paratuberculosis (316F) had low levels of Ara, Gal and Man with major
- monosaccharides being Glc and two unidentified monosaccharides. A second
- M. paratuberculosis strain (J10) had a single TLC band containing only
- Glc. These known strains were compared to two slow growing mycobacterial
- isolates, one from a Crohn's patient and one isolated from armadillo.
- These were similar to J10 in only having Glc present: the former also
- had a similar NMR spectrum to J10, whereas the latter had a different
- NMR spectrum from any of the other strains analysed. The results
- therefore indicate that M. paratuberculosis strain 316F is more closely
- related to M. avium (from an AIDS patient) than it is to the classical
- M. paratuberculosis strain J10 and a Crohn's isolate.
- DE AIDS-Related Opportunistic Infections/MICROBIOLOGY Chromatography, Gas
- Chromatography, Ion Exchange Chromatography, Thin Layer Crohn
- Disease/COMPLICATIONS/MICROBIOLOGY Glycolipids/*CHEMISTRY Human
- Monosaccharides/ANALYSIS Mycobacterium avium
- Complex/*CHEMISTRY/CLASSIFICATION/ISOLATION & PURIF Mycobacterium
- avium-intracellulare Infection/COMPLICATIONS/ MICROBIOLOGY
- Mycobacterium paratuberculosis/*CHEMISTRY/CLASSIFICATION/ ISOLATION &
- PURIF Nuclear Magnetic Resonance
- Paratuberculosis/COMPLICATIONS/MICROBIOLOGY Species Specificity
- JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
-
-