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1996-01-30
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Document 0520
DOCN M9610520
TI Novel glycolipids of Mycobacterium avium and related M. paratuberculosis
strains of relevance to AIDS and Crohn's disease.
DT 9601
AU Ohene-Gyan KA; Haagsma J; Davies MJ; Hounsell EF; Department of
Bacteriology, Central Veterinary Institute,; Lelystad, The Netherlands.
SO Comp Immunol Microbiol Infect Dis. 1995 Jun;18(3):161-70. Unique
Identifier : AIDSLINE MED/96002248
AB The polar glycolipid fractions of several mycobacterial strains of the
closely related species M. avium and M. paratuberculosis have been
analysed by thin layer chromatography (TLC), high pH anion exchange
chromatography (HPAEC), gas-liquid chromatography (GC) and nuclear
magnetic resonance (NMR) spectroscopy. The upper phase of a Folch
partitioning (rather than the lower phase analysed by others) was
subjected to TLC in solvent system chloroform-methanol-water 50:40:10
v/v/v. A major band was purified from each mycobacterial strain.
Monosaccharide analysis of that from M. avium A14 (from an AIDS patient)
contained Glc, Ara, Man, Gal in ratios 7:4:3:2. whereas one strain of M.
paratuberculosis (316F) had low levels of Ara, Gal and Man with major
monosaccharides being Glc and two unidentified monosaccharides. A second
M. paratuberculosis strain (J10) had a single TLC band containing only
Glc. These known strains were compared to two slow growing mycobacterial
isolates, one from a Crohn's patient and one isolated from armadillo.
These were similar to J10 in only having Glc present: the former also
had a similar NMR spectrum to J10, whereas the latter had a different
NMR spectrum from any of the other strains analysed. The results
therefore indicate that M. paratuberculosis strain 316F is more closely
related to M. avium (from an AIDS patient) than it is to the classical
M. paratuberculosis strain J10 and a Crohn's isolate.
DE AIDS-Related Opportunistic Infections/MICROBIOLOGY Chromatography, Gas
Chromatography, Ion Exchange Chromatography, Thin Layer Crohn
Disease/COMPLICATIONS/MICROBIOLOGY Glycolipids/*CHEMISTRY Human
Monosaccharides/ANALYSIS Mycobacterium avium
Complex/*CHEMISTRY/CLASSIFICATION/ISOLATION & PURIF Mycobacterium
avium-intracellulare Infection/COMPLICATIONS/ MICROBIOLOGY
Mycobacterium paratuberculosis/*CHEMISTRY/CLASSIFICATION/ ISOLATION &
PURIF Nuclear Magnetic Resonance
Paratuberculosis/COMPLICATIONS/MICROBIOLOGY Species Specificity
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).