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- Document 0046
- DOCN M9620046
- TI In vivo proviral burden and viral RNA expression in T cell subsets of
- patients with human T lymphotropic virus type-1-associated
- myelopathy/tropical spastic paraparesis.
- DT 9602
- AU Cho I; Sugimoto M; Mita S; Tokunaga M; Imamura F; Ando M; First
- Department of Internal Medicine, Kumamoto University School; of
- Medicine, Japan.
- SO Am J Trop Med Hyg. 1995 Oct;53(4):412-8. Unique Identifier : AIDSLINE
- MED/96074151
- AB We used in situ hybridization combined with immunocytochemistry, cell
- sorting, and the polymerase chain reaction (PCR) to investigate clinical
- events in three asymptomatic carriers of human T lymphotrophic virus
- type-1 (HTLV-1) and ten patients with HTLV-1-associated
- myelopathy/tropical spastic paraparesis (HAM/TSP). The objective was to
- determine which T cell subset of peripheral blood mononuclear cells
- (PBMC), CD4 or CD8, were infected by HTLV-1 and the manner in which
- HTLV-1 proviral DNA was expressed at the level of the single cell. Both
- CD4-positive and CD8-positive cells of the PBMC from five patients with
- HAM/TSP were infected with HTLV-1. The proportion of HTLV-1-infected
- cells was 2.5-40% in the CD4-positive subset and 1.0-65% in the CD8-
- positive subset, when quantified by PCR using HTLV-1-infected MT2 cells
- as a positive standard. Proviral DNA of HTLV-1 was expressed in both
- CD4-positive cells and CD8-positive cells of the PBMC from six patients
- with HAM/TSP and three asymptomatic HTLV-1 carriers. In patients with
- HAM/TSP, the proportion of the cells expressing HTLV-1 proviral DNA was
- 0.02-0.1% in both subsets. In asymptomatic carriers, the expression of
- HTLV-1 proviral DNA was 0.01-0.02% in the CD4-positive subset and 0.01%
- in the CD8-positive subset. Therefore, HTLV-1 possessed similar in vivo
- cellular tropism for both CD4-positive cells and CD8-positive cells and
- HTLV-1 proviral DNA was expressed in vivo in both circulating T cell
- subsets.(ABSTRACT TRUNCATED AT 250 WORDS)
- DE Adult Aged Aged, 80 and over Base Sequence Cell Separation DNA
- Primers/CHEMISTRY DNA, Viral/BIOSYNTHESIS/CHEMISTRY Female Flow
- Cytometry Gene Expression Regulation, Viral Human
- HTLV-I/GENETICS/*PHYSIOLOGY Immunohistochemistry In Situ Hybridization
- Male Microspheres Middle Age Molecular Sequence Data Paraparesis,
- Tropical Spastic/*VIROLOGY Polymerase Chain Reaction
- Proviruses/GENETICS/*PHYSIOLOGY RNA, Messenger/BIOSYNTHESIS RNA,
- Viral/*BIOSYNTHESIS T-Lymphocyte Subsets/*VIROLOGY JOURNAL ARTICLE
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
-
-