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M9620653.TXT
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1996-02-26
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Document 0653
DOCN M9620653
TI Detection of HIV-1 proviral sequences in lymphocytes using a qualitative
polymerase chain reaction assay.
DT 9602
AU Marin MG; Lillo F; Varnier OE; Bresciani S; Molinelli A; Abecasis C;
Bonini PA; Albertini A; Institute of Chemistry, School of Medicine,
University of; Brescia, Spedali Civili, Italy.
SO Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):621-5. Unique Identifier
: AIDSLINE MED/96047093
AB The performance and clinical relevance of a qualitative PCR-based assay
for the detection of HIV-1 DNA sequences in peripheral blood mononuclear
cells (PBMCs) was evaluated by two different laboratories. Four hundred
and one samples were obtained from 397 individuals from different risk
populations. All blood donors tested had negative results; positive
signals were obtained from all infected patients. HIV-1 DNA was detected
in 3 of 17 infants born to seropositive mothers; Western blot
indeterminate blood donors and exposed health-care workers had negative
results. Our results demonstrate that this PCR assay provides both
sensitive and specific results and is suitable for testing large numbers
of samples and for rapid identification of HIV-1 infection.
DE Base Sequence DNA, Viral/*ANALYSIS Human HIV-1/GENETICS/*ISOLATION &
PURIF Lymphocytes/*VIROLOGY Molecular Sequence Data *Polymerase Chain
Reaction Support, Non-U.S. Gov't JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).