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1996-03-30
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Document 0927
DOCN M9650927
TI Development and ultrastructure of Trachipleistophora hominis n.g., n.sp.
after in vitro isolation from an AIDS patient and inoculation into
athymic mice.
DT 9505
AU Hollister WS; Canning EU; Weidner E; Field AS; Kench J; Marriott DJ;
Department of Biology, Imperial College of Science, Technology; and
Medicine, London, UK.
SO Parasitology. 1996 Jan;112 ( Pt 1):143-54. Unique Identifier : AIDSLINE
MED/96155214
AB Continuous culture was achieved in several cell lines of a
microsporidium obtained from the skeletal muscle of an AIDS patient.
Development in COS-1 and RK13 cells was prolific. Spores from the
original biopsy were also inoculated into athymic mice by i.m. and i.p.
routes. Infection was found in several organs as well as in skeletal
muscle after a few weeks. All stages were surrounded by an
electron-dense surface coat. Meronts had 2-4 nuclei and divided by
binary fission. In sporogony the surface coat became separated from the
plasma membrane to form a sporophorous vesicle, within which division
into sporoblasts was effected by repeated binary fissions. The number of
sporoblasts (and later spores) within the sporophorous vesicles varied
from 2 to > 32 and the sizes of the vesicles varied, according to the
number of spores contained therein, from 5 microns diameter to 14.0 x
11.0 microns. Spores measured 4.0 x 2.4 microns and had a prominent
posterior vacuole. The parasite differs from the genus Pleistophora in
that it does not form multinucleate sporogonial plasmodia and that the
sporophorous vesicle enlarges during sporogony and its wall is not a
multilayered structure. It is proposed to place it in a new genus and
species Trachipleistophora hominis n.g., n.sp.
DE Animal AIDS-Related Opportunistic Infections/*PARASITOLOGY Cell Line
Human Mice Mice, Inbred BALB C Mice, Nude Microspora
Infections/COMPLICATIONS/*PARASITOLOGY
Microsporida/CLASSIFICATION/GROWTH & DEVELOPMENT/*ISOLATION &
PURIF/ULTRASTRUCTURE Muscles/CYTOLOGY/PARASITOLOGY Support, Non-U.S.
Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).