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M9610705.TXT
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1996-01-30
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Document 0705
DOCN M9610705
TI Quantification of feline immunodeficiency virus (FIV) proviral DNA in
peripheral blood mononuclear cells of cats infected with Japanese
strains of FIV.
DT 9601
AU Inoshima Y; Tomonaga K; Ikeda Y; Miyazawa T; Mikami T; Department of
Veterinary Microbiology, Faculty of Agriculture,; University of Tokyo,
Japan.
SO J Vet Med Sci. 1995 Jun;57(3):487-92. Unique Identifier : AIDSLINE
MED/96031673
AB The polymerase chain reaction (PCR) method was applied for measurement
of the proviral DNA copy number of feline immunodeficiency virus (FIV)
in peripheral blood mononuclear cells (PBMC) of cats experimentally and
naturally infected with FIV. In experimentally infected cats except one
cat infected with the Petaluma strain, FIV-specific DNAs were
efficiently amplified with the PCR method under the conditions used in
this study. In the naturally FIV-infected cats, the specific DNAs were
also amplified. We established a quantitative method for measurement of
proviral DNA copy number in PBMC from cats infected with TM2-type of FIV
strains, and found that the number was variable among the six cats
examined, ranging from 10(4.0) to 10(5.7) copies per 10(5) PBMCs. This
method can be applicable to cats naturally infected with FIV of
TM2-type. Proviral DNA quantitation developed here could be useful as an
additional parameter to evaluate the relationships among the proviral
load, immune response and development of the clinical symptoms, and to
monitor efficacy of antiviral therapy in vivo.
DE Animal Antibodies, Viral/*BLOOD Base Sequence *Cat Diseases Cats
Comparative Study CD4-CD8 Ratio DNA Primers DNA, Viral/*BLOOD
Enzyme-Linked Immunosorbent Assay/METHODS Feline Acquired
Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY/ VIROLOGY
Gamma-Globulins/ANALYSIS Immunodeficiency Virus, Feline/*ISOLATION &
PURIF Lymphocytes/*VIROLOGY Molecular Sequence Data Polymerase Chain
Reaction/METHODS/VETERINARY Proviruses/*ISOLATION & PURIF Sensitivity
and Specificity JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).